Biomimetic experiment of enoyl-reduction process by F420-dependent enzyme obtained from Saccharopolyspora erythraea and the biosynthetic implication
| Main Author: | Perpustakaan UGM, i-lib |
|---|---|
| Format: | Article NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 1997
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/23334/ http://i-lib.ugm.ac.id/jurnal/download.php?dataId=6279 |
Daftar Isi:
- To approach the involvement of 5-deazaflavin coenzyme (F420) on the enoyl-reduction process in the step 4 of the erythronolide B in Saccharopolyspora erythraea, an investigation was carried out using biomimetic experiment. Three a,b¬unsaturated-bearing substances, i.e. ethyl crotonate, citral, and dimethyl glutaconate, were chosen as the hydride-acceptor substances in the experiment to mimic the step 4 intermediate: 7,9-dihydroxy-2,4,6,8-tetramethy1-5-oxo-undec-2-enoyl-S¬ACP. In the experiment crude extract isolated from Sac. erythraea was used to catalyze the hydride transfer from the NADPH, as the hydride donor, to the acceptors through F420. The hydride transfer was indicated by A401 of the coenzyme, before and after the transfer process which was under dim light and oxygen free condition. The results showed that coenzyme F420 has a role in the enoyl-reduction process of an a,b-unsaturated carbonyl structure. Keywords: Saccharopolyspora erythraea � F420 � enoyl-reduction � biomimetic experiment.