RAPID CONFIRMATION OF RABIES ANTIGEN IN FORMALIN-FIXED PARAFIN-EMBEDDED TISSUE BY IMMUNOPATHOLOGIC DIAGNOSIS

Main Author: Perpustakaan UGM, i-lib
Format: Article NonPeerReviewed
Terbitan: [Yogyakarta] : Universitas Gadjah Mada , 1996
Subjects:
Online Access: https://repository.ugm.ac.id/22062/
http://i-lib.ugm.ac.id/jurnal/download.php?dataId=4942
Daftar Isi:
  • ABSTRACT: Skunks experimentally infected with street virus rabies intramuscularly were used to demonstrate rabies viral antigen in paraffin-embedded tissue by immunopatholoa diagnosis approach, the avidin biotin complex (ABC) method. The use of ABC method provides a simple and sensitive method to localize rabies virus antigen in formalinfixed tissues. The results of the present study also demonstrated that a procedures using formalin-fixed parafin-embeded tissue and stained immunopathologically by the ABC method was an excellent method for both preservation of morfological details of cells/tissues and demonstration of rabies virus antigen.