Soybean protoplast isolation and culture. (Pemisahan dan Pembiakan Protoplas Kedelai)
| Main Author: | Perpustakaan UGM, i-lib |
|---|---|
| Format: | Article NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 1992
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/21932/ http://i-lib.ugm.ac.id/jurnal/download.php?dataId=4811 |
Daftar Isi:
- Protoplas kedelai dipisahkan dari kotiledon muda (15 � 20 hari bunga mekar) yang direndam selama 5 jam di dalam larutan ensim dengan kandungan selulose 2%. Pembelahan sel teram0 selang 3 hari sesudah pembiakan di dalam media cair KP8, apabila protoplas diikat dalam alginat berdaya lekat. lemah dengan kadar 1%. Varietas Galunggung nampak lebih unggul dibandingkan dengan varietas lainnya dalam hasil protoplas dan kemampuan membelah. Soybean protoplasts were isolated from immature cotyledons (15 � 20 days after anthesis) incubated for 5 hours in the enzyme solutions containing 2% cellulase. Cell division was observed after 3 days incubation in the KP8 liquid medium when the protoplasts were embedded in 1% low viscosity of alginate. Galunggung voriety was superior both in the protoplast yield and plating efficiency.