Uji hipersensitivitas kontak dan spesifisitas terhadap merkuri (Hg) pada tikus Wistar
| Main Author: | Perpustakaan UGM, i-lib |
|---|---|
| Format: | Article NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 1998
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/20735/ http://i-lib.ugm.ac.id/jurnal/download.php?dataId=3591 |
Daftar Isi:
- ABSTRACT Mercury is one of the potent allergens which can induce contact hypersensitivity. This substance however, is commonly used for cosmetics and medical purposes. The exact mechanisms underlying the mercury-induced hypersensitivity remain unclear. Studies on mercury contact hypersensitivity in human were hampered due to ethical reason. This investigation was, therefore, aimed at studying hypersensitivity reactions In animal model. Thirty five female Wistar rats, aged three months were divided into three groups of treated animals and two groups of controls. The treated groups were sensitized with 2,5%, 5%, and 10% HgCl2 In petrolatum for 10 days. At day 14, animals were challenged with 5% HgCl2 in 97% ethanol. Contact hypersensitivity was assessed by measuring the ear swelling before and after ear challenge. Specificity test was carried out by challenging the sensitized animals with K2Cr207. The results showed that the, peak levels of ear swelling could be achieved by applying 10% HgCl2 as compared to other groups of animals. Chromium (K2Cr207) ear challenge following 10% HgC12 sensitization failed to induce ear swelling. It can be concluded that the mercury hypersensitivity reaction in this animal model is antigen-specific. Key words: allergy - contact hypersensitivity-mercury - DTH reaction - Wistar rats Di Indonesia merkuri (Hg) masih banyak dipakai sebagai campuran pada bahan kosmetik dan restorasi gigi. Di lain pihak merkuri merupakan alergen yang poten untuk menimbulkan hipersensitivitas. lmunopatogenesis hipersensitivitas kontak (HK) terhadap merkuri belum diketahui secara pasti. Untuk mempelajari HK terhadap merkuri sulk dilakukan pada manusia secara tuntas karena alasan etika. Penelitian Ini bertujuan untuk mempelajari HK terhadap merkuri pada binatang model. Digunakan tikus Wistar umur 3 bulan sebanyak 35 ekor yang dibagl menjadi 5 kelompok yaitu 2 kelompok kontrol dan 3 kelompok perlakuan. Tiga kelompok perlakuan masing-masing dilnduksi dengan salep HgCl2 2,5%, 5% dan 10% selama 10 had. Pada had ke 14 dilakukan paparan ulang pada daun telinga dengan larutan HgCl2 5%. Sebelum dan sesudah paparan ulang tebal telinga diukur dengan mikrometer. Uji spesifisitas reaksi dilakukan dengan cara memberi paparan ulang K2Cr207 pada tikus yang sebelumnya dlinduksi dengan HgCl2. Dad hasil penelitian int dapat dislmpulkan bahwa pengolesan merkuri pada kulit tikus Wistar dapat menimbulkan reaksi hipersensitivitas kontak. Reaksi tertinggi dicapal setelah pemberian salep HgC12 10%. Reaksi hipersensitivitas kontak terhadap merkuri pada tikus Wistar bersifat spesifik antigen.