Molecular evidence of gene integration in Larix decidua somatic embryos after agrobacterium-mediated transformation
| Main Author: | Perpustakaan UGM, i-lib |
|---|---|
| Format: | Article NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2001
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/18201/ http://i-lib.ugm.ac.id/jurnal/download.php?dataId=979 |
Daftar Isi:
- Agrobacterium was considered to be the method of choice to introduce foreign genes into plant cells. ESM (embryonal suspensor masses) which is composed of actively dividing immature embryos, could probably be very competent for transformation. An average of 1- 3 clump tissues resistant to hygromycin arose from at least 300 mg ESM of Larix decidua after Agrobacterium inoculation. These clump tissues were able to proliferate further in the selection medium. PCR analysis was carried out using primers corresponding to HPT and GUS-genes. Both primers amplified an internal fragment in size of 650 and 700 base pair lengths respectively. Keywords: conifer � Larix decidua � Agrobacterium -mediated transformation � embryonal suspensor masses