Restriction fragment length polymorphism analysis of PCR-amplified 16S rDNA of 2.4-D-degrading bacteria isolated form corals
| Main Author: | Perpustakaan UGM, i-lib |
|---|---|
| Format: | Article NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2000
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/18117/ http://i-lib.ugm.ac.id/jurnal/download.php?dataId=895 |
Daftar Isi:
- Identification of 2,4-D-degrading bacteria isolated from corals was carried out based on restriction analysis. Five endonucleases (Hhal, HaeIII, Sau3 AI, Rsal and MspI) were used to digest PCR-amplified 16S rDNA of these bacteria, and produced several informative bands. Comparing all the restriction patterns among four 2,4-D-degrading bacteria, two genotypes (I and II) were obtained. Based on 16S rDNA sequence analysis, those bacteria were identified as Vibrio natriegens and Vibrio alginilyticus. Keywords: 2,4-D-degrading bacteria � 16S rDNA � PCR � RFLP � phylogenetic