Cloning and expression of choline dehydrogenase gene (betA) of escherichia coli in yeast saccharomyces cerevisiae
| Main Author: | Perpustakaan UGM, i-lib |
|---|---|
| Format: | Article NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2000
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/17949/ http://i-lib.ugm.ac.id/jurnal/download.php?dataId=724 |
Daftar Isi:
- The complete DNA sequence of betA, encoding choline dehydrogenase of Escherichia coli has been cloned in a yeast expression-secretion vector and expressed in Saccharomyces cerevisiae DBY 746. The recombinant plasmid (designated as pYYF9) was stably maintained in yeast cells at a level of 24% after 30 generations. Gene expression analysis was carried out at transcription and translation levels. The niRNA analysis by DNA-RNA hybridisation using a specific betA probe did not reveal any signal of transcription of the cloned betA gene in yeast. Protein analysis by SDS-PAGE did not show any specific protein secreted into the yeast supernatant either. However, analysis of the int racellular proteins of yeast demonstrated differences of protein profile between the untransformed with the transformed cells. Keywords : choline dehydmgenase gene (betA) --gene expression � Saccharomyces cerevisiae