PCR amplification and cloning of otolerance gene of rhizobacteria
| Main Author: | Perpustakaan UGM, i-lib |
|---|---|
| Format: | Article NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2000
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/17937/ http://i-lib.ugm.ac.id/jurnal/download.php?dataId=712 |
Daftar Isi:
- A novel isolate of rhizobaeteria which demonstrated osnwtolera nee properties has been obtained. A set of primers for amplification of betA gene, encoding choline dehydnwenase which is involved in the osmotolerance properties in Escherichia cob, has been used to amplifil a similar gene in the rhizobacterial isolate. Following PCR amplification, a single DNA fragment of kb was obtained. The amplification fragment was subsequently cloned into pUC19 plasnzid, resulting in a recombinant plasmid designated as pUC19-R.16. Hybridisation analysis using a probe developed for betA gene demonstrated that the cloned DNA fragment had a little degree of similarity with betA gene of E. coll. Keywords osmotolerance rhizobacteria. ing glycine betaine.