Optimization of random amplified polymorphic DNA technique to reveal genetic diversity of sugarcane germplasm
| Main Author: | Perpustakaan UGM, i-lib |
|---|---|
| Format: | Article NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 1999
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/17805/ http://i-lib.ugm.ac.id/jurnal/download.php?dataId=573 |
Daftar Isi:
- Random amplified polymorphic DNA (RAPD) technique was used to evaluate genetic diversity of 80 randomly selected sugarcane (Saccharum officinarum L.) accessions. Factors affecting polymerase chain reaction (PCR)-RAPD were optimized to obtain high resolution and stable/repeatable DNA banding patterns. DNA amplification by using RAPD technique was sensitive to annealing temperature and time, dNTPs and Mg2* concentrations. Pure DNA was essential for obtaining a high resolution and stable/repeatable DNA banding patterns. Primer annealing occurred at multiple regions, allowing detection of polymorphism using a single primer. Forty two decamer primers were tested and yielded 11 primers which gave highly polymorphic DNA bands that could be used for analyzing genetic diversity of the accessions. Genetic similarity of the accessions was estimated and a dendogram was constructed using the unweighted pair group method with arithmatic average (UPGMA). A high degree of genetic diversity was found among the accession, indicating the possible use of RAPD markers for the characterization and identification of sugarcane germplasm. Keywords: Saccharum officinarum -molecular markers - genetic distance - phylogenetic.