Ekspresi dan respon imun kandidat vaksin DNA dari gen gag-Ca virus penyakit Jembrana=Expression and immune response of DNA Vaccine Candidate of Jembrana disease virus Gag-Ca Gene
| Main Author: | Perpustakaan UGM, i-lib |
|---|---|
| Format: | Article NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2006
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/17767/ http://i-lib.ugm.ac.id/jurnal/download.php?dataId=532 |
Daftar Isi:
- ABSTRACT: Jembrana disease is a disease which specifically attack Bali cattle. The causative agent of disease is a virus from family of Retroviridae and subfamily of development of DNA vaccine candidate, psDNA-CA, was produced by inserting to gag-ca gene which code the t apsid (CA) antigen of the virus to an eukaryotic expression vector, pcDNA3.1. The objectives of this research were to study the expression of gag-ca gene in mammalian cells and to study the immune response that was elicited by DNA vaccine. The gag-ca gene expression was examine by DNA transfection to mammalian cell culture (Hela cell line). DNA transfection was done by co-precipitation technique of DNA-Calcium phosphate in 2X HeBS buffer. The gag-ca expressions were analyzed at the level of mRNA synthesis by RT-PCR (Reverse Transcription Polymerase Chain Reaction) and at the level of protein synthesis by Western Immunoblotting. The immune response which produced the specific antibody was measuring by ELISA technique to Balb/c mice serums, which its were injected intramuscularly by the DNA plasmid with the dosage of 100 ug each at 0, 3 and 6 week. Serums were collected at two and four weeks after the last vaccination. The result showed that the construction of DNA vaccine candidate expressed the gag-ca gene in Hela cell culture either mRNA or CA protein type. The result of vaccination showed that mice were vaccinated by pcDNA-CA produced antibodies against CA antigen. Keywords : Jembrana disease virus, DNA vaccine, gag-ca gene, gene expression, antibody response.