Cloning of betain aldehyde dehydrogenase gene of Escherichia coli and its use as a probe for related gene in salt-tolerant rhizospheric microorganisms
| Main Author: | Perpustakaan UGM, i-lib |
|---|---|
| Format: | Article NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 1999
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/17761/ http://i-lib.ugm.ac.id/jurnal/download.php?dataId=526 |
Daftar Isi:
- Betain aldehyde dehydrogenase gene (betB) of Escherichia coli has been PCR-amplified and cloned into pUC19 plasmid. The amplified fragment was subsequently labeled with digoxigenin and used as a probe for hybridization with either the genomic DNA of E. coli as well as with the rhizospheric microorganisms genome. The labeled probe has successfully detected the betB gene in the E. coli genome. The same probe, however, gave no positive signal when used for detection of related gene in the genome of several osmotolerant rhizospheric microorganisms isolates. Keywords: Betain aldehyde dehydrogenase � glycine betaine � rhizospheric microorganisms