Ekspresi Protein Rekombinan dari Gen gag-ca sebagai Calon Vaksin Virus Penyakit Jembrana = Expression of Recombinant Protein from gag-ca Gene for Jembrana Disease Virus Vaccines Candidate
| Main Author: | Perpustakaan UGM, i-lib |
|---|---|
| Format: | Article NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2004
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/17616/ http://i-lib.ugm.ac.id/jurnal/download.php?dataId=375 |
Daftar Isi:
- Jembrana disease virus (JDV) is the agent of an acute infectious Jembrana disease (JD) of Bali cattle (Bos javanicus), reported for the first time in Jembrana village in Bali, Indonesia. Jembrana disease is not unique to Bos javanicus. Other types of cattle are also susceptible although the resulting lesions are milder when compared to a case fatality rate of about 17 % in Bos javanicus. The aim of this research is to get CA protein and from pGEX-CA expression plasmid that to be expressed in Escherichia coli BL21 after be cultured in 2X YTA medium with IPTG addition at 37°C. pGEX-CA recombinant plasmid that be isolated from Escherichia coli DH5a to be transformed in Escherichia coli BL21. Transforman bacteria than to be cultured in 2X YTA medium with different time and concentration IPTG addition at 37°C. The result of this research are CA fusion protein 48 kDa optimally expressed in 2X YTA medium with IPTG induction 1 mM at OD600 0,6 at temperature 37°C. Key words : Jembrana Disease Virus, CA recombinant protein, protein expression.