Produksi Artemisinin melalui Kultur Pucuk Artemisia cina Berg ex Poljakov = The Artemisinin Production by Shoot Culture Artemisia cina Berg ex Poljakov
| Main Author: | Perpustakaan UGM, i-lib |
|---|---|
| Format: | Article NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2003
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/17390/ http://i-lib.ugm.ac.id/jurnal/download.php?dataId=142 |
Daftar Isi:
- Artemisinin was a secondary metabolite that had potential effect as an antimalarial compound. In Artemisia annua, Artemisinin presented as a sesquiterpene lacton internal peroxide, which had an effective activity as antimalarial agent against certain strain of Plasmodium sp. Considered as A. cina was a plant belonged to family Asteraceae as well as A. annua, artemisinin source. Research in artemisinin content in A. cina from shoot culture had not been reported. The aim of this research were:(I) to know the ability of A. cina shoot culture to produce artemisinin, (ii) to asses the effect of concentration of the yeast extract, sucrose and their combination to promote the production of artemisinin, (iii) to determine the highest artemisinin concentration produced by shoot culture in the medium containing different concentration of yeast extract, sucrose and their combination. The factorial of randomized complete block design (RCBD) used in this experiment. The first factors were concentration of sucrose (1%, 3%, 5%, 7%). The second factors were concentration of yeast extract (0 mg/1, 100 mg/1, 200 mg/1, 300 mg/1). HPLC was used to analyse quantitatively the artemisinin production. Analysis of variance (ANOVA) and DMRT were used to analyse the data. The results showed that yeast extract and sucrose increased the concentration of artemisinin from the A. cina. The highest artemisinin produce was 14.035-mg/g dry weights which was produced by combination of 3 % sucrose concentration and 300 mg/1 yeast extract. This result suggest that shoot culture could be used as a method to produce artemisinin from A. cina. Keywords: Artemisinin -sucrose -yeast extract