Sekuensing Gen Melanocortin 1 Receptor (MC1R) pada Sapi Bali Betina Kupang
| Main Authors: | Tabun, Arnold Christian, Hartatik, Tety, Sumadi, Sumadi, Volkandari, Slamet Diah, Soewandi, Bayu Dewantoro Putro, Penu, C. Leo |
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| Format: | Proceeding PeerReviewed application/pdf |
| Bahasa: | eng |
| Terbitan: |
, 2014
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/139224/1/C28_%20A%20C%20Tabun%20%282014%29_Semnas_Rev.pdf https://repository.ugm.ac.id/139224/ |
Daftar Isi:
- The aim of this research was to identify the nucleotide sequence of the Melanocortin 1 Receptor gene in sorrel-, black- and white- Bali cows by PCR, DNA sequencing, and the percentage of nucleotide similarity. Blood sampels were taken from seventeen sorrel-, eighteen black-, and eight white Bali cows. Identification of nucleotode used PCR and DNA sequencing method. Amplification of PCR product to the MC1R-gene of Bali cow-originated-Kupang was at 296 bp.Nucleotide have change is p52C>T, p223C>A in cattle (Sorrel, white Bali cows) and p147C>T in cattle (black, sorrel and white Bali cows). Nucleotide C plays an important role in the formation of black pigment, changes nucleotide C to nucleotide T causes change in coat color at sorrel and white Bali Cows. It can be concluded that the melanocortin 1 receptor gene play a role in the formation of coat color in Bali cow Kupang with a percentage of nucleotide similarity were 99.662% at black Bali cow, Sorrel and white Bali cows were 98.986% with Bos taurus