Preliminary study on inulin extraction from local Indonesian tubers
| Main Authors: | Kusmiyati, Nur, Wahyuningsih, Tutik Dwi, Widodo, Widodo |
|---|---|
| Format: | Proceeding NonPeerReviewed application/pdf |
| Bahasa: | eng |
| Terbitan: |
, 2016
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/139126/1/Mbak%20mia%20ICST%20lengkap.pdf https://repository.ugm.ac.id/139126/ |
Daftar Isi:
- The aim of this experiment was to investigate various experimental purification methods to determine which obtained the highest percentage of inulin extract. We performed inulin extraction, physicochemical analyses, determination of inulin concentration by high performance liquid chromatography (HPLC), Fourier transform infrared spectroscopy (FTIR) and H nuclear magnetic resonance (NMR) analyses in order to test each of the four methods. Of the tested methods, simultaneous ultrasonic/microwave-assisted extraction (UMAE) produced the highest concentration of inulin compared with the stirring extraction, ultrasound-assisted extraction and indirect sonication methods. The extracted inulin was mainly composed of total sugar (73.22%), whereas fat, ash, protein and reducing sugars were observed only in small quantities. The highest concentration of dietary fibre was obtained from UMAE (47.97%), therefore, UMAE was selected for further analysis. The FTIR analysis of inulin from the tuber produced similar results to the inulin standard, and was determined to contain 11.0923% by HPLC analysis.