Detection and Transcription of Homolog Genes Encoding Conjugated Linoleic Acid in Lactobacillus casei Strains Isolated from Gasterointestinal Tract
| Main Authors: | Sukarno, Ari Surya, Widianto, Donny, Widodo, Widodo |
|---|---|
| Format: | Proceeding NonPeerReviewed application/pdf |
| Bahasa: | eng |
| Terbitan: |
, 2016
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/139125/1/Ari%20surya%20ICST%20lengkap.pdf https://repository.ugm.ac.id/139125/ |
Daftar Isi:
- Conjugated linoelic acid (CLA) is bioactive compound that can be synthesized by probiotic. Lactobacillus casei strain AP and AG were isolated from Indonesian infant and had a potential as probiotic culture. The objective of this study was to detect and measure level transcription of the genes responsible for CLA synthesis in Lactobacillus casei. The gene detection was performed by amplification of parsial genom in conserve location of cla-hy, cla-dh, and cla-dc genes from Lactobacillus plantarum AKU1009a. Bacterial culture was grown in media with and without addition of linoleic acid (0.4 mg/ml). The transcription of cla-hy, cla-dh, and cla-dc homolog genes were analyzed by quantitative Real Time Polymerase Chain Reaction (qRT-PCR). Amplification products in L. casei strain AG was 143, 206, and 148 bp respectively. These sequences were identified as parsial cla-hy, cla-dh, and cla-dc homolog genes. The same primer did not amplify cla-hy, cla-dh, and cla-dc genes in L. casei strain AP. Level of transcription of cla-hy, cla-dh, and cla-dc homolog gene in L. casei strain AG grown in media containing linoleic acid 0.4 mg/ was 0.74; 0.69; and 1.43 fold change respectively, and this was not significant (P>0.05). The transcription of cla-hy, cla-dh, and cla-dc homolog genes in L. casei strain AG was not induced by addition of linoleic acid at 0.4 mg/ml.