Anthelminthic Efficacy of Gliricidia Sepium, Calliandra Calothyrsus, and Artocarpus Heterophyllus by in Vitro Meansurement Against Haemanchus Contortus Worm
| Main Authors: | Kustantinah, Kustantinah, Setyono, W., Dono, N. Danar, Orskov, E.R. |
|---|---|
| Format: | Article PeerReviewed application/pdf |
| Bahasa: | eng |
| Terbitan: |
, 2014
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/139052/1/img527.pdf https://repository.ugm.ac.id/139052/ |
Daftar Isi:
- This study was conducted to determine the efficacy of some herbaceous forage that contain tannin as anthelminthic for sheep against Haemanchus Contortus Worm. The forage used were Gliricidia Sepium, Calliandra Calothyrsus, and Artocarpus Heterophyllus. In current study, female Haemanchus Contortus worms were isolated from sheep abomasum. In Vitro study was done using immersion technique with the concentrations of forage infusion of 0, 10, 30, and 50% (w/v) in a 4x4 factorial arrangement. A screening method was performed afterwards to axamine anthelmintic capacity of the tested forages by calculating the number of paralyzed or dead worms. Ten female Haemanchus Contortus worms were placed in a petri dish containing 10 ml forage infusion for each concontration, according to the treatment. Observations of paralysed or dead worns were done in room temperature every 15 minute in the first hour and continued subsequently every hour in the next 5 hours. Paralysed worms were determined as from weak worms to completely unmoved ones. Results of current study indicated that the increase in length of immersion time and concentration of forage infusion increased (P<0.05) the number of paralysed or dead worms. Infusion of Calliandra Calothyrsus, and Artocarpus heterophyllus showed higher (P<0.05) efficacy when compared to those of Gliricidia. Started at concentration 30% Calliandra and Artocarpus affect mortality of the worms. Artocarpus Heterophyllus showed the higher mortality effect when compared to others leaves (P<0.05). this could be attributed to the tannin content of Artocarpus leaves, which is 2.49% (DM), compared to Calliandra Calothyrsus (1.20% DM) and Gliricidia Sepium (0.60%; DM). It can be concluded that, by in vitro evaluation. Artocarpus, Gliricidia and calian