The effect of adding vitamin c and e in native chicken semen extender storaged at temperature 4 °c on semen quality and egg fertility
| Main Authors: | Asmarawati, Widya, Ismaya, Ismaya, Yuwanta, Tri |
|---|---|
| Format: | Proceeding NonPeerReviewed application/pdf |
| Bahasa: | eng |
| Terbitan: |
, 2010
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/137849/1/ISTAP%202010%20%28Ismaya-34%29.pdf https://repository.ugm.ac.id/137849/ |
Daftar Isi:
- The objective of this research was to verify the addition of vitamin E (a-tocopherol) and vitamin C (ascorbic acid) as antioxidants and the storage at 4°C on semen quality and fertility. Semen was collected and pooled once time every 3 days from 10 cockerels aged 56 weeks. It was diluted into Buffer phosphate and devided into seven (7) treatments, i.e ; control; vitamin E (atocopherol 0.1 mM, 0.3 mM, 0.5 mM), and vitamin C (ascorbic acid 2,500 pg/mL; 5,000 pg/mL; 7,500 pg/mL), and then was stored at 4°C for 72 hours. Semen quality was evaluated after 0 hour, 6 hour, 24 hour, 48 hour, and 72 hours storage. Data were analyzed by analyses of variance split subject, and Duncan’s Multiple Range Test (DMRT). A total of 24 hens of Lohman Brown commercial layers were inseminated with 400 x 106 sperm for chicken fertility trials. The result showed that diluents treatment gave significantly effect (P<0.05) on pH and sperm abnormality, but not different on motility and viability. Stored sperm gave significantly effect (P<0.05) on motility, pH, viability, and sperm abnormality. Vitamin E improved the fertilizing ability until 93.10 ± 5,98 % of semen stored for 6 hours at 4°C at the dose of 0,3 mM of semen diluent. It can be concluded that addition of vitamin E to extended chicken semen keeps motility, viability, and sperm normal morphology after 72 hours storage at 4°C. In contrast, vitamin C decrease motility, pH, and sperm viability. Key words: native chicken, buffer phosphate, vitamin C, vitamin E, semen quality, fertility