In vitro fresh sperm preparation for maintaining sperm viability at storage temperature of 14°C using tannin supplementation of lamtoro leaves
| Main Authors: | Mirajuddin, Mirajuddin, Kustono, Kustono, Ismaya, Ismaya, Budiyanto, Agung |
|---|---|
| Format: | Proceeding NonPeerReviewed application/pdf |
| Bahasa: | eng |
| Terbitan: |
, 2010
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/137848/1/ISTAP%202010%20%28Ismaya-33%29.pdf https://repository.ugm.ac.id/137848/ |
| ctrlnum |
137848 |
|---|---|
| fullrecord |
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<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><relation>https://repository.ugm.ac.id/137848/</relation><title>In vitro fresh sperm preparation for maintaining sperm viability at storage temperature of 14°C using tannin supplementation of lamtoro leaves</title><creator>Mirajuddin, Mirajuddin</creator><creator>Kustono, Kustono</creator><creator>Ismaya, Ismaya</creator><creator>Budiyanto, Agung</creator><subject>Animal Reproduction</subject><description>A research on in vitro fresh sperm preparation for maintaining sperm viability at storage temperature of 14°C using tannin supplementation of lamtoro leaves (Leucaena leucocephala) had been carried out in both Physiology and Animal Reproduction Laboratory, Faculty of Animal Science, Universitas Gadjah Mada for fresh sperm preparation and sperm quality evaluation, during March to July of 2009.The experiment was designed using the Complete Random Design consisted of 5 treatments and 5 replications. Variables measured were viability, motility and morphology of the treated fresh sperm stored for 14 days at temperature of 14°C. Based on the analysis of variance, the treatments had significant effect (P<0.05) on viability, motility and morphology of the sperm. In
terms of viability, Pl(64.07%±0.45) it was higher than control (P0=26.71%±0.54), but it was not different for P2(57.44%±0.52), P3(49.49%±0.71), and P4 (47.83%±0.61). The progress and very progress motility of spermatozoa were only found in control, and not in others. Non-progressive spermatozoa proportion ofPI, P2, P3 and P4,was found respectively. They were significantly higher
than control.For the morphology, P4 had lower normal spermatozoa percentage than P0, PI, P2 and P3, whereas among P0, PI, P2, and P3, there were not different (P>0,05).
Key words: fresh sperm preparation, sperm quality, viability, tannin, Lamtoro leaves</description><date>2010-10-19</date><type>Journal:Proceeding</type><type>PeerReview:NonPeerReviewed</type><type>File:application/pdf</type><language>eng</language><identifier>https://repository.ugm.ac.id/137848/1/ISTAP%202010%20%28Ismaya-33%29.pdf</identifier><identifier> Mirajuddin, Mirajuddin and Kustono, Kustono and Ismaya, Ismaya and Budiyanto, Agung (2010) In vitro fresh sperm preparation for maintaining sperm viability at storage temperature of 14°C using tannin supplementation of lamtoro leaves. In: The 5th ISTAP. International Seminar on Tropical Animal Production: Community Empowerment and Tropical Animal Industry, October 19-22, 2010, Yogyakarta. </identifier><recordID>137848</recordID></dc>
|
| language |
eng |
| format |
Journal:Proceeding Journal PeerReview:NonPeerReviewed PeerReview File:application/pdf File |
| author |
Mirajuddin, Mirajuddin Kustono, Kustono Ismaya, Ismaya Budiyanto, Agung |
| title |
In vitro fresh sperm preparation for maintaining sperm viability at storage temperature of 14°C using tannin supplementation of lamtoro leaves |
| publishDate |
2010 |
| topic |
Animal Reproduction |
| url |
https://repository.ugm.ac.id/137848/1/ISTAP%202010%20%28Ismaya-33%29.pdf https://repository.ugm.ac.id/137848/ |
| contents |
A research on in vitro fresh sperm preparation for maintaining sperm viability at storage temperature of 14°C using tannin supplementation of lamtoro leaves (Leucaena leucocephala) had been carried out in both Physiology and Animal Reproduction Laboratory, Faculty of Animal Science, Universitas Gadjah Mada for fresh sperm preparation and sperm quality evaluation, during March to July of 2009.The experiment was designed using the Complete Random Design consisted of 5 treatments and 5 replications. Variables measured were viability, motility and morphology of the treated fresh sperm stored for 14 days at temperature of 14°C. Based on the analysis of variance, the treatments had significant effect (P<0.05) on viability, motility and morphology of the sperm. In
terms of viability, Pl(64.07%±0.45) it was higher than control (P0=26.71%±0.54), but it was not different for P2(57.44%±0.52), P3(49.49%±0.71), and P4 (47.83%±0.61). The progress and very progress motility of spermatozoa were only found in control, and not in others. Non-progressive spermatozoa proportion ofPI, P2, P3 and P4,was found respectively. They were significantly higher
than control.For the morphology, P4 had lower normal spermatozoa percentage than P0, PI, P2 and P3, whereas among P0, PI, P2, and P3, there were not different (P>0,05).
Key words: fresh sperm preparation, sperm quality, viability, tannin, Lamtoro leaves |
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Universitas Gadjah Mada |
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Perpustakaan Pusat Universitas Gadjah Mada |
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DAERAH ISTIMEWA YOGYAKARTA |
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2017-02-25T15:12:33Z |
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2017-02-25T15:12:33Z |
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dc |
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1765816684492357632 |
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17.538404 |