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Selection of Human-origin Lactobacillus strains as Probiotics with Capability in Synthesizing Conjugated Linoleic Acid and Alleviating Hyperglycemia in Rats (Rattus norvegicus) in vivo

Main Authors: Widodo, Widodo, Harsita, Pradiptya Ayu, Anindita, Nosa Septiana, Wahyuningsih, Tutik Dwi, Nurrochmad, Arief
Format: Proceeding PeerReviewed application/pdf
Bahasa: eng
Terbitan: , 2015
Subjects:
Food technology
Online Access: https://repository.ugm.ac.id/135791/1/Gabung%20ISTAP.pdf
https://repository.ugm.ac.id/135791/
ctrlnum 135791
fullrecord <?xml version="1.0"?> <dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><relation>https://repository.ugm.ac.id/135791/</relation><title>Selection of Human-origin Lactobacillus strains as Probiotics with Capability in Synthesizing Conjugated Linoleic Acid and Alleviating Hyperglycemia in Rats (Rattus norvegicus) in vivo</title><creator>Widodo, Widodo</creator><creator>Harsita, Pradiptya Ayu</creator><creator>Anindita, Nosa Septiana</creator><creator>Wahyuningsih, Tutik Dwi</creator><creator>Nurrochmad, Arief</creator><subject>Food technology</subject><description>The objective of this study was to select Lactobacillus strains as potential probiotics with the capability to synthesize bioactive compounds Conjugated Linoleic Acid (CLA) and their ability to alleviate hyperglycemia in rats (Rattus norvegicus) in vivo. Five strains of Lactic Acid Bacteria consists of Lactobacillus casei strain AP and AG, and Pediococcus acidilactici strains AA, BE and BK were previously isolated and identified from faeces of infants who consumed breast milk as the only source of diet. In vitro evaluation those five strains showed their potential as probiotic based on their capability to grow on media with pH 2.0 and 1.5% concentration of bile salts, the ability to attach on gastric mucin in vitro, and their ability to inhibit the growth of pathogen. Evaluation on the ability to use prebiotic inulin as carbon source showed that Lactobacillus casei (strain AP and AG) and Pediococcus acidilactici strain BE had the ability to degrade inulin as a prebiotic. Evaluation of probiotic on their capability to synthesize Conjugated Linoleic Acid (CLA) from free linoleic acid showed that Lactobacillus casei strain AP was able to convert more than 60% of free linoleic acid to CLA in the media. Further in vivo studies using rats (Rattus norvegicus) showed that Lactobacillus casei strain AP had the ability to alleviate hyperglycemia. The ability of Lactobacillus casei strain AP in reducing hyperglycemia was comparable with that of metformin (anti-hyperglycemia drug) provided orally at level 45 mg/kg of body weight.</description><date>2015</date><type>Journal:Proceeding</type><type>PeerReview:PeerReviewed</type><type>File:application/pdf</type><language>eng</language><identifier>https://repository.ugm.ac.id/135791/1/Gabung%20ISTAP.pdf</identifier><identifier> Widodo, Widodo and Harsita, Pradiptya Ayu and Anindita, Nosa Septiana and Wahyuningsih, Tutik Dwi and Nurrochmad, Arief (2015) Selection of Human-origin Lactobacillus strains as Probiotics with Capability in Synthesizing Conjugated Linoleic Acid and Alleviating Hyperglycemia in Rats (Rattus norvegicus) in vivo. In: The 6th ISTAP International Seminar on Tropical Animal Production, 20-22 ctober 2015, Yogyakarta Indonesia. </identifier><recordID>135791</recordID></dc>
language eng
format Journal:Proceeding
Journal
PeerReview:PeerReviewed
PeerReview
File:application/pdf
File
author Widodo, Widodo
Harsita, Pradiptya Ayu
Anindita, Nosa Septiana
Wahyuningsih, Tutik Dwi
Nurrochmad, Arief
title Selection of Human-origin Lactobacillus strains as Probiotics with Capability in Synthesizing Conjugated Linoleic Acid and Alleviating Hyperglycemia in Rats (Rattus norvegicus) in vivo
publishDate 2015
topic Food technology
url https://repository.ugm.ac.id/135791/1/Gabung%20ISTAP.pdf
https://repository.ugm.ac.id/135791/
contents The objective of this study was to select Lactobacillus strains as potential probiotics with the capability to synthesize bioactive compounds Conjugated Linoleic Acid (CLA) and their ability to alleviate hyperglycemia in rats (Rattus norvegicus) in vivo. Five strains of Lactic Acid Bacteria consists of Lactobacillus casei strain AP and AG, and Pediococcus acidilactici strains AA, BE and BK were previously isolated and identified from faeces of infants who consumed breast milk as the only source of diet. In vitro evaluation those five strains showed their potential as probiotic based on their capability to grow on media with pH 2.0 and 1.5% concentration of bile salts, the ability to attach on gastric mucin in vitro, and their ability to inhibit the growth of pathogen. Evaluation on the ability to use prebiotic inulin as carbon source showed that Lactobacillus casei (strain AP and AG) and Pediococcus acidilactici strain BE had the ability to degrade inulin as a prebiotic. Evaluation of probiotic on their capability to synthesize Conjugated Linoleic Acid (CLA) from free linoleic acid showed that Lactobacillus casei strain AP was able to convert more than 60% of free linoleic acid to CLA in the media. Further in vivo studies using rats (Rattus norvegicus) showed that Lactobacillus casei strain AP had the ability to alleviate hyperglycemia. The ability of Lactobacillus casei strain AP in reducing hyperglycemia was comparable with that of metformin (anti-hyperglycemia drug) provided orally at level 45 mg/kg of body weight.
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