BITING FLIES AS VECTORS OF TRYPANOSOMIASIS AND THE ROLE OF ONE HEALTH IN ANIMAL HEALTH
| Main Authors: | Susmiati, Trini, Widayanti, Rini, Satriyo, Pamungkas Bagus |
|---|---|
| Format: | Article PeerReviewed application/pdf |
| Bahasa: | eng |
| Terbitan: |
Fakultas Kedokteran Hewan Universitas Gadjah Mada
, 2014
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/135265/1/certificate.pdf https://repository.ugm.ac.id/135265/ http://fkh.ugm.ac |
Daftar Isi:
- Chicken is a local Indonesian germplasm that need to be explored its potential. Currently there are several clumps has specific characteristics and some has potential for commercial poultry and for tourism purposes. Ontilnow ,supplyingpoultrynative to Indonesiais still a problem, because of the unavailabilityof commercialstrainswiththe performancestandard, sothere is nocommercialbreedingspecialized in providinglocalpoultry.ldentification and characterization efforts of kedu chicken and local chicken is still needed, it is considered that this activity is·very important as well as it is useful for Indonesiangermplasm conservation, helping original Indonesian chicken breeding programs and helpthe programs for genetic resources conservation (genetic diversity) Indonesian chicken. Effortsto preserve thenativeof Indonesianchicken,andthe power ofnatural selectionwill determine thesurvival ofoffspringtoreproductive processes. Whilethe selectionis madecan bechosenbased onexcellenceforhuman needs. This study usedlocal chickenandkeduchicken fromTemanggungCentral Java.Bloodwas takentoisolateDNAusingconventionalmethods, phenolchloroform(Sambrooket al, 1996).DNAmoleculeswereobtainedamplifiedby PCR-RAPD andresultdielectrophoresisusing3% agarose. Polymorphisms ofDNA bandselectrophoresisresultswere analyzedtoseethe geneticdiversitybetween localchickenandkedu chicken.The results of RAPD-PCR using primers 3, 4, 5, and 6 indicate the presence of genetic diversity within populations and among populations, but can not be used as genetic markers. The closest genetic distance is KeduBlirik to KeduMerah and KeduPutih; as well as the KeduMerah andKeduPutih, and the most distant is local chicken and KeduHitam. Keywords: Kedu chicken, local chicken, PCR-RAPD. 135