AKTIVITAS ANTIINFLAMASI FRAKSI TIDAK LARUT N-HEKSANA DARI EKSTRAK DIKLOROMETANA DAUN Plantago lanceolata L. DAN ANALISIS EKSPRESI COX-2
| Main Authors: | , RINI SULISTYAWATI, , Dr. rer. nat. Nanang Fakhrudin, M.Si., Apt. |
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| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2014
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/133838/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=74697 |
Daftar Isi:
- Inflammation is a local response of living tissue to an injury. Prostaglandin that formation catabolized by the cyclooxygenase enzyme is one of the mediators released on inflammation. It is necessary to look for an agent that can affect the regulation of the cyclooxygenase enzyme. From previous studies it is known that the dichloromethane extract of Plantago lanceolata leaves inhibits NF-kB inflammatory mediators and activate PPARs in vitro in HEK293 cells (Human Embryonic Kidney) and inhibits leukocyte migration. Therefore, in this study, we assessed whether the insoluble fraction of n-hexane from dichloromethane extract Plantago lanceolata (FTLH) leaves antiinflammatory activity in vivo and in vitro. Research on anti-inflammatory activity of standarized FTLH has done by looking at the volume of edema, expression of COX-2 and inhibition of COX-1 and COX-2 to assess selectivity. Mice were divided into 6 groups. Negative group control induced carrageenan on the left hind paw and solvent oils given orally, positive group induced carrageenan and given indomethacin 37.5 mg /kgBW and Na diclofenac at dose of 19.5 mg /kgBW, test group were treated FTLH at doses 39 mg /kgBW, 78 mg/kgBW and 156 mg/kgBW. The paw volume was measured with plethysmometer at 0 hr and for 6 hours after treatment. The removal of tissue on the foot for further immunohistochemical staining to determine COX-2. Inhibition of COX-1 and COX-2 was conducted by Colorimetric COX (ovine) Inhibitor Screening Assay kit (Cayman). The results showed FTLH provide anti-inflammatory activity with power value respectively by (22.84± 6,52)%