ANALISIS ANTOSIANIN DAN KANDUNGAN NUTRIEN PADA BEKATUL BERAS HITAM (Oryza sativa L. cv cempo ireng Sayegan, Yogyakarta)
| Main Authors: | , PRATIWI. A., , Dr. Rumiyati, M.Si.,Apt |
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| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2014
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/133299/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=73880 |
Daftar Isi:
- Black rice is becoming more popular and recently is consumed as a functional food that may provide health benefits, due to its contain such as anthocyanin, vitamins, and minerals. The information of the potential black rice bran of cultivars local in Indonesian very limited. The aim of this study is to analyze anthocyanins, antioxidant activity and analysis of nutrient content from black rice bran culivar local Sayegan, Yogyakarta. Viscous extract of bran black rice obtained by maseration. Analysis of anthocyanin are carried out by separation of viscous extract using preparative TLC silica gel F254 stationary phase and mobile phase B:A:W (4:1:5). The study of fraction using standard of cyanidin 3-O-glucoside with analytical TLC cellulose stationary phase and mobile phase acetic acid: HCl: water (30:3:10) was obtained values of fraction 1 at 0.56 Rf, fraction 2 at 0.71 Rf and standard cyanidin 3-O-glucoside at 0.56 Rf. The study of fractions was continued with HPLC mobile phase of 0.1% phosphoric acid:acetonitril (80:20) gradient. Retention time of fraction 1 was obtained at minute 4.44, while fraction 2 at minute 6,79. Based on HPLC analysis, fractions 1 continued analysis with LC-MS. The molecular weight of fraction 1 m/z 449.10 and fragment ions showed that the fraction 1 can be tentatively identified as cyanidin 3-Oglucoside. The level of cyanidin 3-O-glucoside in this study was 66,1±10,6 Î1⁄4g/g fresh weight. Activity of DPPH free radical scavenging was obtained (IC50) at 200.960 Î1⁄4g/ml fraction 1 and fraction 2 at 218.361 Î1⁄4g/ml respectively. The analysis of macronutrients in this study consists of reduced-unreduced carbohydrate, proteins, and fats at levels 12.56 ± 1.49%