Pengaruh polimorfisme C677T gena methylentetrahydrofolate reductase (MTHFR) terhadap kejadian relaps pada anak dengan Leukemia limfoblastik akut (LLA)
| Main Authors: | , DENNY WELLYAM SIGARLAKI, , Dr. dr. Sri Mulatsih, SpAK |
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| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2014
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/133243/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=73807 |
Daftar Isi:
- Methotrexate is a key drug in acute lymphoblastic leukemia (ALL) treatment. It inhibits DNA replication by blocking the conversion of 5,10 methylenetetrahydrofolate to 5-methylenetetrahydrofolate by methylene tetrahydrofolate reductase, a critical enzyme involved in folate metabolism, DNA methylation and synthesis. It has two common functional polymorphism (C677>T and A1298>C). Genetic polymorphisms of this enzyme have been shown to impact relapse risk in childhood ALL. This study aimed to asses the impact of MTHFR polymorphisms C677>T on the relapse of childhood ALL. This case-control study was undertaken to analyze the presence of the C677>T mutant alleles in 36 (relapse group) and 36 (no relapse group) childhood ALL patients. Polymorphisms in MTHFR C677>T were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The frequency of polymorphism are higher in the control (19% vs 8,8%), but not significantly different (OR 0,379