PENGEMBANGAN DAN VALIDASI METODE PENETAPAN KADAR NATRIUM DIKLOFENAK PAD A SPIKED PLASMA MANUSIA DAN SEDIAAN TABLET SECARA KROMATOGRAFI CAIR KINERJA TINGGI
| Main Authors: | , Fathul Jannah, , Dr. rer.nat. RR. Endang Lukitaningsih, M.Si., Apt |
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| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2014
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/133240/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=73804 |
Daftar Isi:
- Diclofenac sodium is an NSAID class of drugs that are often used for all sorts of pain, gout, rheumatoid arthritis, osteoarthritis or other inflammatory conditions. The use of diclofenac sodium will possibly combined with other drugs, such as thiamine. This study aims to develop and validate an HPLC method for setting the levels of diclofenac sodium in plasma spiked and tablet dosage and see the intervention of thiamine to the determination of diclofenac sodium. HPLC system were developed using RP-18 column LiChrospher in LiChroCART (125 mm x 4 mm, 5 Î1⁄4m), the mobile phase a mixture of methanol and 0.12% acetic acid (65:35 v/v) with a flow rate of 1.0 mL / min, detection was performed at a wavelength of 281 nm. Validation methods include: selectivity, linearity, precision, accuracy, LOD, LOQ. Validation results showed that the method developed selective, have repeatability, precision between, and good accuracy and has a linear standard curve in the range of 1 â�� 6 mg / mL with r = 0.9997, for the determination of diclofenac sodium levels in the spiked plasma and the tablet dosage