PENGARUH KONSENTRASI EKSTRAK KULIT BATANG JAMBU METE SEBAGAI DASAR BAHAN KUMUR TERHADAP SITOTOKSISITAS SEL FIBROBLAS DENGAN METODE MTT
| Main Authors: | , AHMED FEBRI NOOR HERTAMA, , Drg. Harsini, M.S. |
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| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2014
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/132002/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=72517 |
Daftar Isi:
- Recurrent aphthous stomatitis is an inflammation that occurs in the oral mucosa. One of the treatment to cure recurrent aphthous stomatitis is by using mouthwash ingredients. Cashew bark contains phenolic compounds that include flavonoids, tannins and saponins. The aim of this study was to determine the effect of variations in the concentration of cashew stem bark extract as the base material mouthwash towards cytotoxic effect on fibroblast cells using MTT method. This material used in this study was cashew stem bark which were extracted by maceration method using 70% ethanol. A total of 15 samples were grouped into 5, each consisting of 3 samples (ISO 10993-5). Concentrations used were 1.6%, 0.8%, 0.4%, 0.2% dan 0.05%. Cytotoxicity test uses the MTT method by comparing optical density (ELISA plate reader) between treated groups with control groups. Cell viability was obtained by comparing treated groups with control groups. Cell viability data was analyzed using one way ANOVA and LSD. Results showed mean of fibroblast cell viability due to various cashew stem bark extract successively from concentrations 1.6%, 0.8%, 0.4%, 0.2% dan 0.05%. With mean 15.35 ± 0.443%, 30.84% ± 1.59, 47.78 ± 8.09%, 65.74% ± 3.20, 74.95 ± 7.26%. ANOVA showed a significant influence various cashew stem bark on cell viability (p<0,05). Results of LSD showed there were significant difference between treated groups except between concentrations 0.2% and 0.05%. In conclusion, there was influence on the various cashew stem bark extract concentrations towards fibroblast cell cytotoxicity.