KEMAMPUAN SEPULUH ISOLAT BAKTERI PEMBINTIL AKAR DALAM PEMBENTUKAN BINTIL AKAR DAN PENAMBATAN NITROGEN PADA TUJUH TANAMAN LEGUM
| Main Authors: | , NORA FANTA, , Ir. Sri Wedhastri, M.S. |
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| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2014
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/131930/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=72441 |
Daftar Isi:
- Root nodule bacteria is a group of soil bacteria that has ability to develop association with legume plants and form the root nodules. The bacteria live in the root system, promote the development of root structure and fix atmospheric nitrogen. Several members of this group which only particular legumes are commonly known as �cross innoculation group�. Such association develops due to some characteristic properties of particular root nodule forming bacteria. The study was aimed at establishing the ability of ten different root nodule forming isolates to develop association with seven different legume plants. Ten isolates used in this work were obtained from the culture collection of Laboratory of Microbiology, Faculty of Agriculture Faculty, Gadjah Mada University. The isolates were challenged to develop root nodule on seven legume plants, i.e : Siratro (Macroptilium atropurpureum), Soybean (Glycine max (L)), Kembang Telang (Clitoria ternatea), Kacang Merah (Vigna angularis), Trembesi (Samanea saman), Sengon Laut (Albizia falcataria) and Lamtoro (Leucaena glauca). The method used for testing the root nodule development was the rhizotron system using zeolit as the growth media, followed by the analysis of nitrogenase activity by employing Acetyelen Reduction Assay (ARA). The results of the study showed that isolates UGM2 (Bacillus cereus/Acinetobacter calcoaceticum), UGM3 (Brevibacillus centrosporus), UGM14 (Azorhizobium caulinodans), UGM42 (Paenibacillus sp.), UGM50 (Agrobacterium tumefaciens/Rhizobium TANU) and THA7 (Bradyrhizobium japonicum) could form nodules on five legumes (Siratro, Soybean, Kembang Telang, Trembesi and Sengon Laut). Isolates UGM29 (Bradyrhizobium sp.), UGM32 (Bradyrhizobium sp.), UGM38 (Bradyrhizobium elkanii), UGM48, UGM49 (Azorhizobium paspali) and USDA122 (Bradyrhizobium japonicum) could form nodules on six legumes (Siratro, Soybean, Kembang Telang, Kacang Merah, Trembesi and Sengon Laut). All isolates of root nodule-forming bacteria demonstrated higher nitrogenase activity in Siratro than other legumes. Isolate UGM50 (Agrobacterium tumefaciens/Rhizobium TANU) had highest nitrogenase activity. The activity was 2,651 mmol N 2 /g nodule/hour. Based on root nodule formation and nitrogen fixation, isolate UGM2 (Bacillus cereus/Acinetobacter calcoaceticum) was a species similar to Acinetobacter calcoaceticum, while isolate UGM50 (Agrobacterium tumefaciens/Rhizobium TANU) was expected as a genus Rhizobium.