Ekspresi Gen CYPlg Aromatase pada Sel Granulosa Kadar Testoteron Estrogen Serum dan Intra Ovarium Tikus Sparague Dawley
| Main Authors: | , BAIQ MULIATIKA ROSMIATI, , Prof. Dr. Sri Kadarsih S, MSc, PhD, AIFM. |
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| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2014
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/131544/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=72040 |
Daftar Isi:
- Background: One of the major function of ovary is secretion steroid hormone such as estrogen and progesterone. Ovary also the source of testosterone and a great amount of progesterone in the luteal phase of ovarian cycle. CYP19 genes influence the process of steroidogenesis which encode the cytochrome P450 aromatase. These genes catalize the final step of estrogen biosynthesis, testosterone and androstenedione into estradiol and estron. Aim: The aims of this study were to explored expression of CYP19 aromatase gene, testosterone, and estrogen levels in ovarian granulosa cells Sprague dawley rats due to administration of estradiol valerate Method: This study used a simple experimental study design. Subjects were 12 female Sprague dawley rats divided into 2 groups: treated group 6 rats and control group 6 rats. Treatment groups getting treatment giving 0,2 mL estradiol valerate which is 20 mg/mL in concentration in drinking water. Mouse blood was taken for further examined levels of testosterone, and estrogen. Ovarian tissue was taken for further examined levels of testosterone, estrogen, and the expression of CYP19 aromatase gene by immunohistochemical. Statistical analysis was performed with unpaired t test. Results: The results showed that the expression of CYP19 aromatase gene after administration of 0,2 mL estradiol valerate which is 20 mg/mL in concentration in drinking water rats for 30 days did not differ significantly (p> 0.05). Testosterone levels in both ovarian tissue and serum were not significantly different (p> 0.05). Estrogen levels in both ovarian tissue and serum were not significantly different (p> 0.05). Conclusion: Giving 0,2 mL estradiol valerate which is 20 mg/mL in concentration in drinking water of rats over 30 days was not or had not lowered the aromatase CYP19 gene expression, estrogen levels in both ovarian tissue and serum, and had not or did not raise testosterone levels in both ovarian tissue and serum.