UJI AKTIVITAS HIPOGLIKEMIK FRAKSI ETANOL DAN RESIDU DEKOKTA HERBA SAMBILOTO (Andrographis paniculata (Burm.f.) Ness) MELALUI AKSI ANTIOKSIDAN DAN PENINGKATAN TRANSLOKASI PROTEIN GLUT-4 PADA TIKUS RESISTEN INSULIN
| Main Authors: | , HADI KURNIAWAN, , Prof. Dr. Agung Endro Nugroho, M.Si., Apt. |
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| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2014
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/130927/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=71360 |
Daftar Isi:
- Unhealthy lifestyle can increase the risk of diabetes mellitus (DM) type 2 insulin resistance. Obesity (hiperlipidemia) conditions which can lead signals disorder of GLUT-4 protein translocation to the membrane of the muscle cells that can stimulate hyperglycemia and hyperinsulinemia. The protracted hyperglycemic causes increased oxidative stress and excessive production of free radicals. This research aimed to evaluate the effect of ethanol fraction and decocta residual of herbaceous sambiloto (Andrographis paniculata (Burm.f.) Nees) which was suspected contain flavonoid as antioxidants and anti- DM type 2 in insulin resistance rats. Animal of DM type 2 insulin resistances were induced by a high fat diet and fructose for 120 days. Insulin resistance conditions were determined by comparing the parameters value of normal animal experiment of insulin resistance and induction of animal experiment with high fat fructose diet. From the results of statistical analyzes by using independent sample t-test on all parameters of insulin resistance showed that a diet of high fat-fructose of rats induction was indicated the insulin-resistant of diabetes mellitus type 2. After experimental animals were clarified as insulin resistant, the animals were treated with fractions of ethanol and decocta residual of herbaceous sambiloto for 14 days, than data and statistic were analyzed. The decreasing of glucose levels of positive control, ethanol fraction with dose 225 and 675 mg/kgBW and decocta residual fraction groups was differed significantly between day 120 to day 127 and 134. Percentage of power hypoglycemic showed decocta ethanol fraction with dose 225 mg/kgBB (63.45±0.67%) had the best pre-prandial of power hypoglycemic whereas postprandial of power hypoglycemic was possessed by decocta ethanol fraction with dose 225 mg/kgBB (68.29±0.63%) and fraction of ethanol decocta with dose 675 mg/kgBB (64.61±0.41%) after 14 days of treatment. While the results of quantification of the protein translocation of GLUT-4 showed that the fraction of the decocta ethanol group with dose 225 mg/kg was able to increase the translocation of GLUT-4 protein significantly. Ethanol fraction and residual decocta fraction have weak antioxidant activity with IC50 successively 632.08±15.92 and 3,870.88±177.12 ppm with total flavonoid levels of each 5.13±0.07 and 2.05±0.02%.