IDENTIFIKASI DAN UJI ANTAGONISME BAKTERI DARI RIZOSFER Mimosa sp. TERHADAP JAMUR AKAR PUTIH Rigidoporus microporus ISOLAT KARET
| Main Authors: | , Budi Setyawan, , Prof. Dr. Ir. Siti Subandiyah, M.Agr.Sc |
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| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2014
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/130805/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=71238 |
Daftar Isi:
- White root disease caused by Rigidoporus microporus is the most important disease on Hevea rubber. Various control methods were recommended to overcome the disease, but could not solve the problem completely yet. The objectives of this study were to explore antagonistic bacterial isolates from Mimosa sp. rhizosphere, assess the antagonism effectiveness and mechanism against R. microporus in vitro and followed by identification. The methodology were exploration activity, direct opposition test against R. microporus, intracellular and extracellular filtrate test, extracellular enzyme activity test and identification of potential bacteria based on morphological, biochemical and molecular by sequencing the 16S rRNA and gyrB gene. Three potential bacteria strains successfully being isolated from Mimosa sp. rizhosphere that were taken from Hevea rubber plantation, which are Pmg-15, Pmg-29 and Pmg-32. Pmg-32 isolate was able to suppress the growth of R. microporus mycelium on Kingâ��s B, NA, TSA and PDA medium. Pmg-15 and Pmg-29 isolates showed inhibition ability on Kingâ��s B and TSA medium. The inhibition mechanism of those three isolates occurred by extracellular filtrates. The activities of Î2-endoglucanase and protease enzyme was shown by Pmg-32 isolate. Intracellular filtrate did not show any significant inhibition activity. Based on molecular identification using 16S rRNA and gyrB genes, Pmg-15 and Pmg-29 isolates classified into Pseudomonas mosselii group, whilst Pmg-32 isolate classified into Bacillus amyloliquefaciens subsp. plantarum group.