KARAKTERISASI MOLEKULER FRAGMEN GEN FUSI VIRUS NEWCASTLE DISEASE ISOLAT ASAL WILAYAH KERJA BALAI PENYIDIKAN DAN PENGUJIAN VETERINER REGIONAL III LAMPUNG TAHUN 2010-2013
| Main Authors: | , Liza Angeliya, , Dr. drh. Michael Haryadi Wibowo, M.P |
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| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2014
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/130667/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=71094 |
Daftar Isi:
- Newcastle Disease (ND) is an infectious poultry disease that caused by ND virus which is belongs to family Paramyxoviridae, genus Avulavirus, and Avian paramyxovirus species. This disease causes a drop in egg production and has high mortality and high cost of control, which cause economic losses in Indonesia and the world. Vaccination is a method for controlling Newcastle disease in Indonesia, however there were still a lot of cases encountered in the field. Molecular data on the ND virus in the working area of Disease Investigation Center Lampung had not been reported. The purpose of this study is to characterize the fragment of fusion gene, which acts as a molecular determinant of pathogenicity of ND viruses. A total of 12 samples from Disease Investigation Center Lampung from 2010 until 2013 were used in this study. The samples consist of 10 samples of cloacal swabs and two samples of internal organs (trachea, lung, esophagus and intestine). The suspension of organs and cloacal swabs were extracted to obtain RNA. The amplification of DNA was conducted with Reverse Transcription Polymerase Chain Reaction (RT-PCR) using primers designed by the Australian Animal Health Laboratory (AAHL) Geelong, Australia. Another primers from Disease Investigation Center Lampung were used to amplify a fragment from the fusion gene, and then followed by gene sequencing. The results showed that ND were positively identified in all of the samples. The result of sequence analysis with MEGA v5.01 software showed that the amino acids composition in the cleavage site showed that the isolates were classified as velogenic virulent strain of Newcastle Disease virus, with sequence patterns of 112RRQKR�F117 and 112RRRKR�F117. According to phylogenic tree analysis, the isolates from Disease Investigation Center Lampung were classified in the genotype VII subtype VIIa and VIId. Genetic distance between the isolates of Disease Investigation Center in 2010-2013 and Indonesian ND viruses that previously reported in nucleotide of fusion gene fragmen position 103-626 ranged from 0 to 8.6 % and homology between 91.4%-100%.