LISAT PLATELET DAN MEDIUM TERKONDISI SEL PUNCA MESENSIMAL UNTUK MEMPERBAIKI KULIT MENUA DINI Kajian In-vitro pada Fibroblas Kulit Manusia Menua Dini Buatan
| Main Authors: | , Yohanes Widodo, dr., Sp.KK(K), , Prof. Dr. dr. Hardyanto Soebono, Sp.KK(K). |
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| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2014
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/130364/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=70785 |
Daftar Isi:
- Background: Solar radiation onto human skin, mainly ultraviolet A (UVA), can stimulate premature aging skin because of its effects on human dermal fibroblasts (proliferations, collagen depositions, migrations, and TGF-Î2 consumptions). A number of evidence demonstrate that Various biological materials such as platelet lysates (PL), Whartonâ��s Jelly Stem Cells Conditioned Media (WJ) and Adipose Derived Stem Cells Conditioned Media (AD), may improve healing process in chronic ulcers Objectives: This study was performed to evaluate potency of various concentration of PL, WJ, and AD in improving skin aging of human dermal fibroblasts. Methods: A quasi experimental design was done in artificially aged human dermal fibroblasts. Fibroblast cultivated more than 3 passages (11-13 years olds boyâ��s foreskins were divided into two groups: Group I consisted of 10 kJ/cm2 UVA induced aged fibroblasts and Group II were normal fibroblasts without UVA irradiation as positive control. Group I were divided into 4 subgroups: subgroup 1â��3 were treated by various concentration of PL, WJ, AD consecutively and subgroup 4 was a negative control. They were cultured in DMEM standard media. After treatments, they were evaluated its proliferations, migrations, collagen depositions, and level of extracellular TGF-Î2. Fibroblast proliferation, migration, deposition was measured by using MTT assay, Scratch assay, Insoluble Sirius Red collagen assay, respectively, while the level of TGF- was tested by using ELISA. Results: Cultivation of aged fibroblasts in 50% PL showed significant (P<0.05) elevation of proliferations, migrations, and collagen depositions compared to negative control, but they did not have reduction of extracellulary TGF-Î2 levels significantly (p>0.05). Cultivation of aged fibroblasts in 25% PL showed elevation of extracellulary TGF-Î2 levels significantly (p<0.05). Proliferations, migrations, and collagen depositions of aging fibroblast culture in 50% PL were: 0.87±0.049 OD