KAJIAN KERAGAMAN GENETIK GEN PENYANDI NADH Dehydrogenase Sub-unit 1 (ND1) PADA Tarsius bancanus bancanus, Tarsius spectrum DAN Tarsius dianae
| Main Authors: | , ISTIQOMAH, , Dr. drh. Rini Widayanti, M.P. |
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| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2014
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/130201/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=70616 |
Daftar Isi:
- Indonesia has a diversity of flora and fauna that is quite varieble. One of the fauna in Indonesia is primate. Many primate species in Indonesia is endengered because of illegal hunting and industrial activities that caused habitat destruction. One of the endangered species is commonly known as Tarsier monkey. Therefore, conservation efforts are needed to preserve the Tarsier. Molecular exploration is one of the efforts to differentiate Tarsius. The purpose of this research is to analyze the genetic diversity in Tarsius bancanus bancanus, Tarsius spectrum and Tarsius dianae based on the encoding gene sequence of NADH Dehydrogenase Sub-unit 1 (ND1). This research was using Tarsier DNA isolated from the muscle tissue of Tarsius bancanus bancanus, Tarsius spectrum and Tarsius dianae. Amplification of ND1 gene by PCR method with ND1 Forward primer and ND1 Reverse primer. This reaction produces amplicon 1278 bp and by multiple alignment showed a nucleotide sequences of ND1 encoding gene with 957 nt in length. The result of sequenced ND1 gene was compared to the other primates from Genbank and be analyzed with the program MEGA version 5.10. The results of the analysis acquired 4 nucleotide sites and 2 amino acid sites that could be used as genetic markers between Tarsius bancanus bancanus, Tarsius spectrum and Tarsius dianae. The average genetic distance of Tarsier based on the nucleotide sequences of ND1 coding gene which was analyzed using Kimura 2-parameter was 15,5%. Filogram using Neighbor Joining method with bootstrap values of 1000 times could differentiate Tarsius bancanus bancanus, Tarsius spectrum and Tarsius dianae.