PRODUKSI SUBSTRAT FERMENTASI BIOETANOL DARI MAKROALGA Eucheuma cottonii MELALUI HIDROLISIS MENGGUNAKAN ENZIM SELULASE
| Main Authors: | , SEKAR PUSPAWATI, , Dr. Wagiman, STP., M.Si. |
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| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2014
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/129108/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=69486 |
Daftar Isi:
- Bioethanol is an alternative fuel made from biomass which is contained sugar component, starch and cellulose. Indonesian potential aquatic comodity to be developed, macro-algae, could be the raw materials resource for alternative fuel to substitute conventional fuels consequently. Eucheuma cottonii macro-algae have a polysaccharide composition that consists of cellulose, carrageenan and agar. Polysaccharide, especially cellulose, could be hydrolyzed into monosaccharide (glucose) chemically or enzymatic, then fermented into bioetanol. This research is an E. cottonii macro-algae implemenation study to produce substrate for bioethanol fermentation with the high reduction sugar component. The raw materials came from Buton district, South East Sulawesi, were in the form of dry seaweed. The procedure of this research: the reduction of seaweed�s size. The next process was hydrolyzing with cellulase enzyme for 24 hours with enzyme consentrate variation (19, 36, and 52 IU) and temperature (40 and 500C). The sugar reduction analysis was done with Nelson-Somogy Method then processed by using SPSS software (real difference test). After that, calculate the cost of hydrolysis (financial aspect). According to the result of research, it was known that E. cottonii seaweed produce bioethanol fermentation substrate with sugar reduction indicator through enzymatic hydrolysis process by using cellulase enzyme. The best condition of enzymatic hydrolysis process within 12 hours was by elaborating cellulase enzyme 36 IU at 500C temperature. It produces degree of sugar reduction 8,045 mg/ml or 0,865%. However, based on statistic analysis through t-test, the result of degree of sugar reduction is not different with hydrolysis through 19 IU cellulase enzyme at 500C which produces degree of sugar reduction 7,937 mg/ml or 0,794%. Financial feasibility analysis of fermentation substrate production is not feasible because the cash flow criteria are minus. On the other side, from the financial criteria, Payback Period value is unlimited. The value of NPV is -(Rp 1,676,367,500,-), B/C Ratio is -3,34, and there is no breakeven on BEP with amount of production (-741,19 sugar reduction gram per year).