Uji Kepekaan Sel Biofilm Isolat Klinik Pseudomonas aeruginosa Penyebab OMSK di RSUP dr. Sardjito Terhadap Gentamicin
| Main Authors: | , ADI BAGUS ANDRIANTO, , dr. Agus Surono, Sp.THT.KL, Ph.D |
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| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2014
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/128230/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=68568 |
Daftar Isi:
- Background : P.aeruginosa is one of many bacteria that can cause Supppurative Chronic Otitis Media. It can form a biofilm which is difficult to treat because it can defend itself against various antibiotic. Objective : The objective of this research was to do a susceptibility test to a biofilm formed by P.aeruginosa on Chronic Suppurative Otitis Media patients against gentamicin in vitro. Method and Material : Design of this research is observational analitic, which is cross sectional. Biofilm susceptibility test against gentamicin done by microbroth dilution method using 96- well microplate. Concentration of gentamicin was 128, 64, 32, 16, 8, 4, 2, 1, 0.5, and 0.25 g/ml. After putting antibiotic, MTT assay is performed. Results of MTT assay is analysed with MBRC50. Results : There is no concentration founded to have absorbance below MBRC50 cut-off point. Gentamicin concentration that come close to MBRC50 cut-off is at 128 g/ml, which can reduce biofilm cell quantity of P.aeruginosa to the point of 48.5%. Conclusion : Pseudomonas aeruginosa biofilm on CSOM patients who came to THT clinic RSUP dr.Sardjito, given the gentamicin in vitro founded to have highest MBRC of 48.5% at concentration of 128 g/ml.