SEMISINTESIS TURUNAN EURIKUMANON DARI EURIKUMANON AKAR PASAK BUMI (Eurycoma longifolia, Jack.), UJI AKTIVITAS ANTIPLASMODIUM DAN KAJIAN HUBUNGAN KUANTITATIF ANTARA STRUKTUR AKTIVITAS
| Main Authors: | , Hanifah Yusuf, Dra. Apt., M.Kes, , Prof. Dr. Mustofa, M.Kes., Apt |
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| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2014
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/127997/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=68320 |
Daftar Isi:
- Eurycomanone is a C20 quassinoid found in pasak bumi roots plant (E. longifolia, Jack.) from family Simarubaceae. Five eurycomanone ester derivatives were synthesized by using starting material eurycomanone from pasak bumi roots and acetyl chloride, butiryl chloride, valeryl chloride, para-methoxybenzoyl chloride and succinic anhydride. The structure of semisynthesis products were determined as eurycomanone triacetate, eurycomanone dibutyrate, eurycomanone monovalerate, eurycomanone dimethoxybenzoate and eurycomanone disuccinate based on their spectroscopic data of UV, IR, NMR and MS. These compounds and eurycomanone itself were evaluated for their antiplasmodial activities against 3D7 chloroquine-sensitive strain of P. falciparum using Candle Jar method. Their cytotoxic activities on Vero cells were assayed using MTT method and their mechanism of action through inhibition of haem polymerization were also determined by Bassilico et al. (1998) method. The antiplasmodial, cytotoxic, and inhibition of haem polymerization activities were analyzed by linear regression method for determining their IC50 values. The prediction for finding new eurycomanone derivatives which are more potential, conducted through analysis of quantitative structure-activity relationship (QSAR) using semiempirical calculation with Austin Model-1 (AM1) method and the best equation gained by multilinear regression analysis. The result of antiplasmodial activity testing have been shown that eurycomanone have IC50 value 0,0047 ± 0,0001