ANALISIS MUTASI A1762T/G1764A DI GENA BASAL CORE PROMOTER PADA PASIEN DENGAN HEPATITIS B KRONIS DAN PENYAKIT HATI TAHAP LANJUT DI RSUP DR SARDJITO YOGYAKARTA
| Main Authors: | , WILLIAM SURYA H., , dr. Didik Setyo Heriyanto, Ph.D, Sp.PA |
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| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2014
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/127992/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=68315 |
Daftar Isi:
- Background: Infection of Hepatitis B Virus (HBV) has been known as one of the major health problems in this world, although, in fact, vaccination and antiviral therapy have also been invented. More than 600.000 people died every year as the consequence of acute and chronic Hepatitis B. Indonesia, as other Asian countries, is a country with moderate-high prevalence of HBV infection. However, studies on characteristic of mutations within HBV genome and their roles in pathogenesis of liver disease in the HBV infected patients have not been conducted in Indonesia. Objective: To investigate the prevalence of basal core promoter mutation, specifically A1762T/G1764 mutation, within patient with chronic hepatitis B and advanced liver disease. Methods: Cross sectional study involved patients with HBV surface antigen (HBsAg) positive. The participants of this study are 40 patients with advanced liver disease, including liver cirrhosis and carcinoma hepatocelullar, and 40 patients with chronic hepatitis B. Cases with positive HBsAg expression for more than six months but did not fulfill the diagnostic criteria of liver cirrhosis or HCC, were diagnosed as chronic HBV infection. Basal core promoter mutation was analyzed by Nested PCR and direct sequencing method. Results: The presence of basal core promoter A1762T/G1764A mutation was observed in 23 (57,5%) patients with advanced liver disease and 10 (25%) patients with chronic hepatitis B. Conclusion: The presence of basal core promoter mutation is significantly higher within advanced liver disease patients, compared with chronic hepatitis B patients. This mutation can be used as a predictive marker for the progression of liver diseases in Indonesia.