BIOAKTIVITAS DAN AKTIVITAS ANTIOKSIDAN EKSTRAK BATANG MAHKOTA DEWA [Phaleria macrocarpa (Scheff) Boerl.]
| Main Authors: | , ANISA ALFIANA GAZIDY, , Dr. Ganis Lukmandaru, S.Hut., M.Agr.Sc. |
|---|---|
| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2013
|
| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/127000/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=67241 |
Daftar Isi:
- Mahkota Dewa [Phaleria macrocarpa (Scheff) Boerl.] is one of herbal plants which is accustomed to use by Indonesian, particularly its fruits, leaves, and barks. Nevertheless, there is no study that have evaluated the chemical properties nor extracts of the stem. This study elucidated the bioactivities and antioxidant activity of mahkota dewa stem which is supported with extractive compound analysis. The base part of the stem was obtained from Depok, Sleman, DI Yogyakarta. The stem powder of the base part in 40 mesh size was extracted successively with n-hexane, ethyl acetate, methanol, and water. The analysis of extractive compound consisted of identification of secondary metabolites by chemical reaction, thin layer chromatography, gas chromatography � mass spectrometry (GC-MS), total phenolic compound, and flavonoid compound by colorimetric method. Bioactivity experiments included antitermitic test to drywood termites (Cryptotermes cynocephalus Light) and Brine Shrimp Lethality Test (BSLT). Antioxidant activity was examined by 1,1-diphenyl-2- picrylhydrazyl (DPPH) method. The analysis of extractive compound by qualitative method showed that terpenoid, steroid, alkaloid, and saponin were detected. Further, the quantitative analysis showed the low level of total phenolic compound while flavonoids were not detected. GC-MS analysis showed the 11 main components. N-hexane and ethyl acetate soluble fractions were toxic to dry-wood termites (termites mortality of 41.67-95.00%) and exhibited the lowest weight loss of paper disc (0.37-1.60 mg). BSLT showed that all fractions were highly toxic and to be potential as an anticancer agent (LC50 � 30 ppm). DPPH method gave the level of antioxidant activity in relatively low inhibition values (7.59-18.12%).