PENGARUH PEMANFAATAN LIMBAH INDUSTRI TEPUNG DAN BAKTERI ASAM LAKTAT TERHADAP PEMBUATAN SILASE TOTAL CAMPURAN PAKAN SERAT

Main Authors: , DIMAS HAND VIDYA PARADHIPTA, , Prof. Ir. Zaenal Bachruddin, M.Sc., Ph.D.
Format: Thesis NonPeerReviewed
Terbitan: [Yogyakarta] : Universitas Gadjah Mada , 2013
Subjects:
ETD
Online Access: https://repository.ugm.ac.id/126612/
http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=66839
Daftar Isi:
  • This research aims were to determine affinity values (KS and Î1⁄4max) of lactic acid bacteria (BAL) to glucose and its application water soluble carbohydrates originated from flour industry waste to improve quality of mixed silage of hay, grass and legumes (total fiber mix silage, STCPS). This experiment consisted of three phases. First phase, selection of the best Lactobacillus among L. plantarum, L.bulgaricus, and L.casei based on the determination of KS and Î1⁄4max by observing the growth of BAL based on dry matter (DM) using limited glucose medium (defined medium), with different levels of glucose (0, 0.01, 0.02, 0.04, 0.06, 0.08, and 0.1%). Second phase, selection of wastes from flour industry as a source of water soluble carbohydrate by determinate the conversion value of water soluble carbohydrate into lactic acid (Yp/s). The best Lactobacillus chosen from phase 1 was grown in a defined medium with substrate sources extract of corn hominy (A), wheat pollard (P), cassava waste (O), corn hominy and wheat pollard mixture (AP), corn hominy and cassava waste mixture (AO), cassava waste and wheat pollard mixture (PO), with the provision of 0.03%. The growth of DM production was observed until the stationary phase was reached, then tested for Yp/s. Third phase, was done to determine the effect of Lactobacillus inoculant and the best waste from flour industry based on KS and Î1⁄4max in the total mix of silage fiber. The inoculant was given at level of 10% and substrate at level of 0KS, 1xKS, and 2xKS. The results showed that L. plantarum was the best Lactobacillus with the lowest KS and the highest Î1⁄4max values (3 mg/100ml and 0,09/h, respectively