BIODEGRADASI PEWARNA AZO ORANGE G DENGAN TEKNIK IMMOBILISASI ISOLAT BAKTERI
| Main Authors: | , YANU ARIYANTI PUSPITA RINI, , Prof. Dr. Ir. Sebastian Margino |
|---|---|
| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2013
|
| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/126270/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=66479 |
Daftar Isi:
- Azo dye are synthetic dyes that widely used in industry, especially textile industry. 7 x 105 � 1 x 106 of dyes are produced annually in the world, which azo dyes represents about 70% by weight. Azo dyes containing one or more azo bond (-N=N-). Estimated that about 10-15% of colorants lost during dyeing process, degradation process is needed to reduce environmental pollution. Degradation prosess using biological methods more effective, because it does not produce secondary pollution, compared to the physical and chemical methods. Azo dye can be degraded by bacterial lignolytic enzyme. The aims of this research was: to isolate lignolytic bacteria that able to degrade azo dye Orange G, to find out the optimum condition of selected isolate in producing lignolytic enzyme, and to determine the ability of immobilized lignolytic enzyme wich applicated on the Orange G. This research was done in steps: (i) lignolytic bacteria isolation, (ii) selection semi quanitative based on the degradation of lignin ability, and selection quantitative based on decolorization of Orange G by biomass and crude enzyme immobilized, (iii) identification of selected isolate, (iv) optimization of lignolytic enzyme production, (v) application immobilized crude lignolytic enzyme on Orange G. Research result showed that as many as 243 isolates were isolated and 56 among them were lignolytic. The ability of degradation lignin by isolates was ranging from 1-10,75. The lignolytic isolates was screened to decolorize Orange G by cell and crude enzyme immobilization. 37,5% and 49,5% decolorization of Orange G was reached by cells immobilization, and 14,91% and 29,18% by lignolytic enzyme. Based on the enzyme activity selection was found that isolate PK.31 was the potential one. Isolate PK.31 was further optimized for fermentation process for lignolytic enzyme production. Analysis of enzyme showed the highest of laccase production was 97,13 U/ml