EVALUASI PEMBENTUKAN BIOFILM, DISTRIBUSI GEN icaA/D SERTA POLA KEPEKAAN ANTIBIOTIK ISOLAT METHICILLIN RESISTEN S. AUREUS (MRSA) DAN METHICILLIN SENSITIF S. AUREUS (MSSA)
| Main Authors: | , TITIK NURYASTUTI, , dr. Praseno, SpMK(K) |
|---|---|
| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2013
|
| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/126194/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=66396 |
| ctrlnum |
126194 |
|---|---|
| fullrecord |
<?xml version="1.0"?>
<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><relation>https://repository.ugm.ac.id/126194/</relation><title>EVALUASI PEMBENTUKAN BIOFILM, DISTRIBUSI GEN icaA/D
SERTA POLA KEPEKAAN ANTIBIOTIK
ISOLAT METHICILLIN RESISTEN S. AUREUS (MRSA)
DAN METHICILLIN SENSITIF S. AUREUS (MSSA)</title><creator>, TITIK NURYASTUTI</creator><creator>, dr. Praseno, SpMK(K)</creator><subject>ETD</subject><description>Nowadays, Staphylococcus aureus, especially MRSA isolates, emerged as a major
pathogenic agent of nosocomial infection and sepsis worldwide. Infections caused by
these bacteria are often very severe and difficult to treat because of the limited
sensitivity of antibiotics and the development of antibiotic resistance is very fast.
Biofilm formation is an important factor in the pathogenicity of Staphylococcus
infections, since the bacteria can attach to and colonizes in biomaterials devices.
Biofilm is one of the reason of antibiotic therapy failure.
In this study, the ability of MRSA isolate (Methicillin Resistant Staphylococcus
aureus) and MSSA (Methicillin Sensitive Staphylococcus aureus) to form biofilm
were studied by congo red agar and microtiter plate test. PCR was performed to detect
the presence of icaA/D, which responsible for biofilm production. Antibiotic
sensitivity was done against MSSA and MRSA isolates by agar diffusion method.
The results of this study showed that 90 % of MRSA isolates were not able to form
biofilm, whereas all MSSA isolates have ability to form biofilm, although all MRSA
and MSSA isolates harbour both genes icaA/D in the chromosomes. MRSA isolates
were more resistant to many classes of antibiotics than MSSA. Antibiotics still potent
for most of the MRSA isolates were chloramphenicol, erythromycin and
cotrimoxazole. There seems to be differences in the mechanisms of regulation and
biofilm formation in MRSA and MSSA isolates. The presence of SSC mec in MRSA
bacterial chromosome, will repress gene controlling of biofilm formation, so MRSA
prefers to express the antibiotic resistance genes which can be used to adapt in clinical
situation.</description><publisher>[Yogyakarta] : Universitas Gadjah Mada</publisher><date>2013</date><type>Thesis:Thesis</type><type>PeerReview:NonPeerReviewed</type><identifier> , TITIK NURYASTUTI and , dr. Praseno, SpMK(K) (2013) EVALUASI PEMBENTUKAN BIOFILM, DISTRIBUSI GEN icaA/D SERTA POLA KEPEKAAN ANTIBIOTIK ISOLAT METHICILLIN RESISTEN S. AUREUS (MRSA) DAN METHICILLIN SENSITIF S. AUREUS (MSSA). UNSPECIFIED thesis, UNSPECIFIED. </identifier><relation>http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=66396</relation><recordID>126194</recordID></dc>
|
| format |
Thesis:Thesis Thesis PeerReview:NonPeerReviewed PeerReview |
| author |
, TITIK NURYASTUTI , dr. Praseno, SpMK(K) |
| title |
EVALUASI PEMBENTUKAN BIOFILM, DISTRIBUSI GEN icaA/D
SERTA POLA KEPEKAAN ANTIBIOTIK
ISOLAT METHICILLIN RESISTEN S. AUREUS (MRSA)
DAN METHICILLIN SENSITIF S. AUREUS (MSSA) |
| publisher |
[Yogyakarta] : Universitas Gadjah Mada |
| publishDate |
2013 |
| topic |
ETD |
| url |
https://repository.ugm.ac.id/126194/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=66396 |
| contents |
Nowadays, Staphylococcus aureus, especially MRSA isolates, emerged as a major
pathogenic agent of nosocomial infection and sepsis worldwide. Infections caused by
these bacteria are often very severe and difficult to treat because of the limited
sensitivity of antibiotics and the development of antibiotic resistance is very fast.
Biofilm formation is an important factor in the pathogenicity of Staphylococcus
infections, since the bacteria can attach to and colonizes in biomaterials devices.
Biofilm is one of the reason of antibiotic therapy failure.
In this study, the ability of MRSA isolate (Methicillin Resistant Staphylococcus
aureus) and MSSA (Methicillin Sensitive Staphylococcus aureus) to form biofilm
were studied by congo red agar and microtiter plate test. PCR was performed to detect
the presence of icaA/D, which responsible for biofilm production. Antibiotic
sensitivity was done against MSSA and MRSA isolates by agar diffusion method.
The results of this study showed that 90 % of MRSA isolates were not able to form
biofilm, whereas all MSSA isolates have ability to form biofilm, although all MRSA
and MSSA isolates harbour both genes icaA/D in the chromosomes. MRSA isolates
were more resistant to many classes of antibiotics than MSSA. Antibiotics still potent
for most of the MRSA isolates were chloramphenicol, erythromycin and
cotrimoxazole. There seems to be differences in the mechanisms of regulation and
biofilm formation in MRSA and MSSA isolates. The presence of SSC mec in MRSA
bacterial chromosome, will repress gene controlling of biofilm formation, so MRSA
prefers to express the antibiotic resistance genes which can be used to adapt in clinical
situation. |
| id |
IOS2744.126194 |
| institution |
Universitas Gadjah Mada |
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19 |
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library:university library |
| library |
Perpustakaan Pusat Universitas Gadjah Mada |
| library_id |
488 |
| collection |
UGM Repository |
| repository_id |
2744 |
| subject_area |
Karya Umum |
| city |
SLEMAN |
| province |
DAERAH ISTIMEWA YOGYAKARTA |
| repoId |
IOS2744 |
| first_indexed |
2016-09-14T18:32:36Z |
| last_indexed |
2016-09-22T21:46:40Z |
| recordtype |
dc |
| _version_ |
1765816521559375872 |
| score |
17.538404 |