Peningkatan Efek Sitotoksik Cisplatin oleh Fraksi Etil Asetat Kayu Secang (Caesalpinia sappan L.) pada Sel HeLa melalui Induksi Apoptosis dan Modulasi Siklus Sel
| Main Authors: | , Ulfatul Husnaa, , Prof. Dr. Edy Meiyanto, M.Si.Apt |
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| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2013
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/125766/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=65942 |
Daftar Isi:
- Cercival cancer evidence is being the third most common cancer in woman worldwide. Globally, 80 to 85% of deaths occur in low-income or middleincome countries. Chemoterapheutic agent uses are found to be effective to tread cervical cancer. The main problem of cisplatin causes significant side effect and cellular resistant problem. New effort is using natural compound with low or moderate toxicity as chemotherapeutic agents of combination with cisplatin with low doses to increase cytotoxic effect of cisplatin. Caesalpinia sappan (C. sappan) is one of potential plant of which the main compounds both of brazilin and brazilein performs cytotoxic effects on cervical cancer cells. Both of compounds soluble in ethyl acetate fraction. The present study was carried out to investigate the cytotoxic activities of ethyl acetate fraction of C. sappan alone and in combination with cisplatin. Cytotoxic activity was evaluated by MTT assay. Cell cycle and cell death analysis was performed by flow cytometry and p53 expression by immunocytochemistry. Ethyl acetate fraction of C. sappan performs cytotoxic effect on HeLa cells with decreasing of cells viability in dose dependent manner, gave IC50 value of 65±1.5Î1⁄4g/ml. Combination of ethyl acetate fraction of C. sappan with cisplatin decreased cell viability up to 36.86%. Further assay indicated that this combination caused cell cycle arrest in S and G2/M phase through p53 stabilization, but not increase apoptosis induction. These results provide scientific evidence that ethyl acetate fraction of C. sappan is potential developed as cochemotherapeutic agent with cisplatin in improving medical effectiveness of cervical cancer. Therefore, further study on its molecular mechanism needs to be done.