PRODUKSI ENZIM PROTEASE DARI Amylomyces rouxii B
| Main Authors: | , ASTRID DIANKA PRATIWI, , Dr. Ir. Retno Indrati, M.Sc. |
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| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2013
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/125337/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=65504 |
Daftar Isi:
- Protease enzyme is one of the most enzyme that classified into hydrolase group, because this enzyme can catalyst the unbounded of peptide bond and have been aplicated through many food or non-food industries. This enzyme can be produce by fungus, one of them is Amylomyces rouxii. A. rouxii has a natural habitat at sticky rice tape fermentation and can be isolated from ragi tape or cassava tape . This study is aim to investigate the production condition of protease enzyme from Amylomyces rouxii. In this work, isolate was incubated in media from sticky rice, corn, or soybean, then the protease activity is measured by solved protein content which is produced by lowry method. Choosen media will be used to investigate the time, the variation of basal salts and temperature effect on enzyme production. The A. rouxii growth in sticky rice at 37oC for 3 days of incubation with variation basal salts MgCl2, produce the highest activity which is 19.57 U/gram media compare to that of anothers.