IDENTIFIKASI PEMALSUAN PRODUK BAKSO SAPI DENGAN DAGING BABI DAN DAGING AYAM MENGGUNAKAN METODE PORCINE DETECTION-KIT DAN MULTIPLEX POLYMERASE CHAIN REACTION
| Main Authors: | , CHRISTINA YUNI ADMANTIN, , Prof.Dr.Ir. Umar Santoso,M.Sc |
|---|---|
| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2013
|
| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/124964/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=65128 |
Daftar Isi:
- Transparancy in meat speciation is very important. Accurate labeling is essential for protection of consumer health and religious credence, also to ensure the authentication of product before they decided to purchase. Pork adulteration and it�s derivates in food product, without any clearly labeling is consider to fraudulent. According to Indonesia legislation, UU No.18/2012, fraudulent in food product might to be subjected by punishment. Besides, most of Indonesian people is Muslim. The presence of pork in food products is seriously concern as pork is banned by Muslim law. The aim of this research was to determine the presence of pork and to identify species authentication in ten meatballs, taken from northern Yogyakarta. Two methods based on protein and DNA were used for identification. They were Porcine Detection KIT and Multiplex Polymerase Chain Reaction. Porcine Detection KIT is based on principle of immunochromatographic rapid test. The target antigens are bound by highly specific antibodies attached to test line and colored microparticles. Multiplex Polymerase Chain Reaction is capable of amplifiying few DNA target into milion copies of DNA, using multiple primers. DNA was isolated from sample using Genomic mini KIT. Optimizing of PCR was conducted in advanced to obtain the most optimum annealing temperatures for DNA amplification. Three sets of primer used in this study for multiplex amplification: pig (Sus scrofa), cow (Bos taurus) and chicken (Gallus gallus). PCR products were analyzed by electrophoresis on 1,5% agarose gel run in TBE1x buffer at 100V. The results showed that Porcine Detection KIT and Multiplex Polymerase Chain Reaction can detect the presence of pork in samples. Porcine Detection KIT can detect one sample which contaminated with pork. Multiplex PCR not only can detect the presence of pork, but also beef and chicken. There were two samples contaminated with pork according to multiplex PCR detection