EKSPRESI COX-2 DAN JUMLAH NEUTROFIL FASE INFLAMASI PADA PROSES PENYEMBUHAN LUKA MUKOSA BUKAL SETELAH PEMBERIAN SISTEMIK EKSTRAK ETANOLIK ROSELA (Hibiscus sabdariffa) ( studiiIn vivo pada Tikus Wistar )
| Main Authors: | , Endah Kusumastuti, , Dr. drg. Juni Handajani, M.Kes., Ph.D |
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| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2013
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/123590/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=63703 |
Daftar Isi:
- Inflammation is an initital stage of body's natural response to tissue damage. One of medicaments to reduce inflammation is nonsteroidal antiinflammatory drugs (NSAIDs). The use of NSAIDs may cause some side effects, and in some ways it may more secure to use medicinal herbs. Roselle (Hibiscus sabdariffa) has potential effect as antiinflammatory agent. This study aimed to know the effect of systemic administration of roselle ethanolic extract toward COX-2 expression and neutrophils number in the inflammatory phase of wound healing processes. Thirty six of male white rats were given to injury on the buccal mucosa by using punch biopsy. The animal models were divided into 3 groups randomly into negative control (saline), positive control (ibuprofen 20 mg/kg), and treatment (roselle 500 mg/kg). Rats were sacrificed on day 1st, 2nd , 3rd and day 4th prior to preparation of buccal mucosa tissues for paraffin blocks. Immunohistochemical staining was performed to determine the expression of COX-2, and hematoxylin eosin staining was done to determine the number of neutrophils. Two-way ANOVA of COX-2 expression and neutrophils number data showed significant difference (p<0.05) in all groups. COX-2 expression and neutrophils number was detected at lowest values on day 4th of roselle-treated group. Clinical observation showed perfect wound closure in roselle-treated group on day 4th. The results demonstrated that the ethanolic extract of roselle could inhibit COX-2 expression and decrease the number of neutrophils. It might be suggested that roselle ethanolic extract can be used as anti-inflammatory agent of wound healing process.