PENGARUH KOMBINASI EKSTRAK TERPURIFIKASI HERBA SAMBILOTO (Andrographis paniculata (Burm.f.) Nees) DAN HERBA PEGAGAN (Centella asiatica (L.) Urban) TERHADAP KADAR GLUKOSA DARAH DAN TRANSLOKASI PROTEIN GLUT-4 PADA TIKUS DIABETES MELLITUS TIPE 2 RESISTEN INSULIN
| Main Authors: | , Novena Yety Lindawati, , Prof. Dr. Agung Endro Nugroho, M.Si., Apt. |
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| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2013
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/123033/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=63143 |
Daftar Isi:
- Sambiloto herb (Andrographis paniculata (Burm. f.) Nees.) and pegagan herb (Centella asiatica (L.) Urban) are excellent medicinal plants which are being developed as traditional for antidiabetic. The research aims to see the effec of combination from purified extract of sambiloto herb and pegagan herb of blood glucose levels and translocation of GLUT-4 protein in model type 2 diabetes mellitus rats-insulin resistance compared with the use of each purified extract. This type of research is experimental. Test preparations were divided into 8 groups include three from combination purified extract of sambiloto herb and pegagan herb: I (912,1: 300), II (651,5: 500), III (390,9: 700) in mg/kg BW, IV (purified extract of sambiloto herb 1303 mg/kg BW), V (purified extract of pegagan herb 1000 mg/kg BW), VI (positive control metformin 45 mg/kg BW), VII (negative control CMC-Na 0.5%) and VIII (normal control). Parameters measured quantitative data at blood glucose levels using colorimetric methods and semi quantitative data translocation of GLUT-4 protein in cells muscles of the thigh (soleus muscle) rats using Immunohistochemistry methods. Test animals are of type 2 diabetes mellitus insulin-resistance was induced by fructose (1,8 g/kg BW) and fat-rich food (95% pellet, 5% egg yolks and 5 mL/ 200 g BW lard) for 70 days. Determination of insulin resistance used 3 parameters such as blood glucose levels, lipid levels (triglycerides, LDL, cholesterol, and HDL), and hypoglikemic tests with glibenklamid compared to normal controls animals. Insulin resistance test results using statistical analysis Independent Samples t-Test showed significant differences (p< 0.05) between normal rats with fatfructose rats at day-50 and -70. This indicates a diet of fat-fructose affects all insulin resistance parameters and fat-fructose rats indicated type 2 DM insulin resistance. Group I-VI appeared decreased levels of blood glucose which significant differences between days 77 and 70 on the analysis of the Paired Sample t-Test. Group of combination I showed effec of blood glucose levels in type 2 DM insulin resistance rats no significant differences (p>0.05) and for translocation of GLUT-4 protein was better than each purified extract with significantly p<0.05 with oneway ANOVA.