AMPLIFIKASI DAN SEKUENSING GEN PENYANDI VIRAL PROTEIN 2 (VP2) CANINE PARVOVIRUS TIPE 2a DARI SPESIMEN DARAH ANJING
| Main Authors: | , NURRIA NOVENDITA, , Dr. drh. Aris Haryanto, M.Si. |
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| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2013
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/122350/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=62452 |
Daftar Isi:
- Canine Parvovirus is the etiology agent of CPV infection that is highly contagious and fatal affecting both wild and domesticated dogs. The CPV is divided into three distinct types that have worldwide distribution, the CPV-2a, CPV-2b, and CPV-2c. The infection causes the severe gastroenteritis, fever, anorexia, lethargy, and death. The objective of this study is to extract the DNA of Canine Parvovirus with the unknown type and then amplified using Polymerase Chain Reaction (PCR) method at the gene encoding the capsid protein VP2. The PCR product obtained then sequenced to determine the nucleotide order of the virus. This study is done to determine the specific type of CPV from fresh blood specimen using PCR and genetic sequencing. The material used in this study is ten fresh blood specimens taken from canine patients admitted in the Prof. Soeparwi Animal Hospital and private veterinary clinics. The specimen then undergone DNA extraction and amplification of the gene encoding the viral protein VP2 with the PCR product length is 518 bp. The positive result then sent for sequencing to determine the order of the nucleotides. The sequencing result then analyzed using MEGA 5.10 and BLAST programs from NCBI. From ten specimens extracted and amplified, one positive sample showed positive result indicated by DNA band on the size of 518 bp. The positive result then sequenced and analyzed. The analysis showed that the sampel obtain was 100% identical to three CPV-2a isolates from GenBank. This concludes that the PCR of the VP2 gene and nucleotide sequencing can be used to determine the specific type of CPV from the fresh blood specimen.