EFIKASI VAKSIN POLIVALEN Aeromonas hydrophila ISOLAT JAWA TIMUR PADA LELE DUMBO (Clarias sp.)
| Main Authors: | , MEEZAN ARDHANU A., , Dr. Ir. Triyanto, M.Si. |
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| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2013
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/122349/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=62451 |
Daftar Isi:
- The aims of this research were to determine the most effective method of vaccination in controlling Motile Aeromonas Septicemia (MAS) in African catfish. The effectiveness vaccination methods were evaluated by survival rate (SR), relative percent survival (RPS), antibody titers, and mean time of death (MTD). The effect of vaccination to specific growth was also observed. This vaccination method consists of three treatments (immersion, oral, and injection) and a control in three replications. Immersion vaccination (P1) was done by dipping the fish in vaccine suspended in water at 10 7 cells/ml for five minutes. Oral vaccination (P2) was done by feeding 0,1 ml vaccine directly through the fish covum oris at a dose of 10 7 cells/fish. Injection vaccination (P3) was done by injecting 0,1 ml of vaccine at a dose of 10 7 cells/fish intraperitoneally. A week after vaccination the second vaccination (booster) was conducted and one week after booster the fish was challenged by intraperitoneally injection of A. hydrophila JTP2 at a dose of LD70 (3.24x10 5 cells/fish). Antibody titers were observed before, after vaccination, and after challenge test. Observations of the survival rate, the relative percent survival, and the mean time of death were done for 14 days after infection. The result showed that the use of polyvalent vaccines increased the survival rate (P <0.05), 40.0-87.5% comparing with control (nonvaccinated), 13.3%. Vaccination also delayed the mean time of death of fishes (P <0,05). The most effective methods was intraperitoneally injection with the values of survival rate reached 87.5% with the relative percent survival level was 86.1%, and the mean time of death was 282 hours (12 days). However, vaccination was not affected on the fish growth rate.