PENGARUH MADU Apis mellifera TERHADAP PERTUMBUHAN BAKTERI PENYEBAB PENYAKIT PERIODONTAL ( Kajian in vitro pada Aggregatibacter actinomycetemcomitans )
| Main Authors: | , ASTARI DWI JAYANTI, , drg. Suryono, S.H., Ph.D, |
|---|---|
| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2013
|
| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/121774/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=61871 |
Daftar Isi:
- The antibacterial power is tested by infusing 50 Âμl Apis mellifera honey at 20%, 30%, 40%, and 50% concentration, Periodontal disease is a disease of periodontal tissue which caused by subgingival plaque bacteria. Aggregatibacter actinomycetemcomitans is a facultative anaerobic gram-negative bacteria that leads to periodontal disease. Apis mellifera honey, derived from Apis mellifera honey bee, is an antibacterial agent which is most commonly found in Indonesia. The aim of this study is to determine the effect of Apis mellifera honey on the growth of Aggregatibacter actinomycetemcomitans bacteria. sterile distilled water as negative control, and 0,2% chlorhexidine as positive control into the well hole of 5 petri dishes (n=5) MHA media planted with Aggregatibacter actinomycetemcomitans. The MHA media were then incubated for 24 hours at 37oC temperature. The inhibition zones formed were measured using caliper. The mean and standard deviation results of the inhibition zones diameter of Apis mellifera honey at 20%, 30%, 40%, and 50% concentration are 2.51 ± 0.439, 3.98 ± 0.599, 5.25 ± 0.220, and 6.55 ± 0.990. Kruskal-Wallis test showed that Apis mellifera honey at 20%, 30%, 40%, and 50% concentration could inhibit the Aggregatibacter actinomycetemcomitans growth. Mann-Whitney test showed there were significant differences among the concentrations. The conclusion of this study is Apis mellifera honey at 20%, 30%, 40%, and 50% concentration are able to inhibit the growth of Aggregatibacter actinomycetemcomitans bacteria.