ISOLASI DAN IDENTIFIKASI VIRUS INFECTIOUS BURSAL DISEASE DENGAN UJI AGAR GEL PRESIPITASI
| Main Authors: | , RADHIYAN FADIAR SAHISTYA, , Dr. drh. M. Haryadi Wibowo, MP. |
|---|---|
| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2013
|
| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/121184/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=61249 |
Daftar Isi:
- Infectious Bursal Disease (IBD) is frequently referred to as Gumboro, is one of acute viral disease, and highly infectious lymphocidal disease of young chickens caused by a double stranded RNA virus. Although IBDV has been isolated from other avian spesies, but the disease is currently recognized in the chicken only. This disease is most of these found in intensive poultry areas in every country and causes economic decrease. Prevention and handling are seriously needed to solve this problem. Isolation and identification of the disease agent certainly conducted in laboratory could give important information to control this disease. This research aimed to find infection of IBD in some poultries in Yogyakarta. The virus was confirmed by Agar Gel Precipitation Test (AGPT). This research used 5 IBD-diagnosed chicken bursal fabrisius samples. The samples was inoculated into 11 day old embryonated chicken eggs through chorioallantoic membrane (CAM). Embryos were monitored for mortality and lesions, then compared with the control sample. While moderate to mild hemorrhaghi were present on the CAM in MHW/Bro/2011, moderate pox were present on the CAM MHW/SR-WN-Layer/2010 and other sample no lesions were observed. Embryos were observed congestion on the cutan, moderate hemorrhages on the cerebral, and delayed feathering in MHW/Yanti-Layer/2011, MHW/SR-WN-Layer/2010, MHW/Bro/2011, and MHW/Bro/2012. In the MHW/Yanti-Layer/2011 and MHW/Bro/2011 were observed hemorrhages on the toe joint. The virus comfirmed by AGPT with samples from cropped embryo and CAM. Samples was concluded positive if showed precipitation reaction at least after 24-48 hours of incubation. Result of research showed that 5 local samples which compared with control samples and confirmed by AGPT, sample (1) MHW/Yanti-Layer/2011, (2) MHW/SR-WN-Layer/2010, (3) MHW/Bro/2011, and (4) MHW/Bro/2012 are positif infected IBD, but sample (1) IBD Potrowang is negative infected IBD. Presipitation were observed from CAM sample, and no presipitation were observed from embryos sample.